Here's a short video of me explaining the Donath Landsteiner test which we use when we are trying to sow thw presence of a biphasic antibody - that is one which binds to antigen in the cold and activates complement in the warmth. These antibodies do not bind in the warmth so antibody panels are often negative.
Sunday, 24 January 2010
Thursday, 14 January 2010
Elutions
The one thing I want to make really sure of is the reason for the last wash and reataining it. You always test your last wash in parallel with your eluate. Only if your last wash shows no reactivity can you say that any antibodies detected in the eluate have come off the cells you are performing the elution on.
The "negativity" of your last wash demonstrates that you have washed away all unbound antibodies from your sample before you perform the elution so, any antibody detected in your eluate must have come from the cells.
Monday, 11 January 2010
Antibody Identification Panels
This is just me trying out some new technology (to me). I'm trying to sort out how to get video of a good enough quality without having the huge file size. So far I've failed and this one is about 52Mb to upload! Apologies all for making such a pig's ear of it over the weekend.
If you want to follow handouts I've already given you then you need to go to the next slide when you hear a guitar ping.
Comments would be really useful!
Monday, 17 August 2009
Bloom's Taxonomy - You Should Know This!
Sunday, 16 August 2009
Email Posting Test
This is just me checking if I can email content from my computer at home to my Transfusion Blog
Friday, 14 August 2009
Sunday, 29 March 2009
Know Your Abs from Your Ags
- Phenotyping - here we are using known antibodies (in the antiserum) to look for the presence / absence of antigens on the patient's cells - antibody known / antigen unknown
- Reverse group (ABO) - we are using the known antigens on the reagent cells (A1rr, Brr and OR1r) to look for the presence / absence of the naturally-occurring anti-A and / or anti-B in the patient's plasma - antibody unknown / antigen known
- Antibody Screen - we are using reagent cells (two or three screening cells) which exhibit the most common antigens to try and detect any atypical red cell antibodies in the patient's plasma - antibody unknown (if present) / antigen known
- Antibody Identification - once we have detected atypical antibodies we test them with a panel of cells where we know their antigen makeup (whether or not each cell has each antigen or not) to identify the antibody / antibodies we have detected - antibody unknown / antigen known
- Serological Crossmatch - we have done the work and avoided antigens to which the patient may adversely react. We now test the cells (antigens) we want to put into the patient as a transfusion with the patient's plasma (antibody) as a last test to see if there are any antibodies in the patient plasma that may react with any antigens on the cells in the blood we will transfuse - antibody unknown / antigen unknown