The Donath Landsteiner Test

Here's a short video of me explaining the Donath Landsteiner test which we use when we are trying to sow thw presence of a biphasic antibody - that is one which binds to antigen in the cold and activates complement in the warmth. These antibodies do not bind in the warmth so antibody panels are often negative.

Elutions

Here's a short video about the elution technique

The one thing I want to make really sure of is the reason for the last wash and reataining it. You always test your last wash in parallel with your eluate. Only if your last wash shows no reactivity can you say that any antibodies detected in the eluate have come off the cells you are performing the elution on.

The "negativity" of your last wash demonstrates that you have washed away all unbound antibodies from your sample before you perform the elution so, any antibody detected in your eluate must have come from the cells.

Antibody Identification Panels

This is just me trying out some new technology (to me). I'm trying to sort out how to get video of a good enough quality without having the huge file size. So far I've failed and this one is about 52Mb to upload! Apologies all for making such a pig's ear of it over the weekend.

If you want to follow handouts I've already given you then you need to go to the next slide when you hear a guitar ping.

Comments would be really useful!