Elutions

Here's a short video about the elution technique

The one thing I want to make really sure of is the reason for the last wash and reataining it. You always test your last wash in parallel with your eluate. Only if your last wash shows no reactivity can you say that any antibodies detected in the eluate have come off the cells you are performing the elution on.

The "negativity" of your last wash demonstrates that you have washed away all unbound antibodies from your sample before you perform the elution so, any antibody detected in your eluate must have come from the cells.